Nanofluidic devices are showing growing interest in the single molecule analysis applications due to their capability to directly probe individual molecules. Single molecule analysis is a powerful approach that allows unprecedented new details of biomedical information. A DNA molecule will stretch out when confined in a nanochannel, linearly arraying the genomic information. The traditional nanochannel based devices are mostly based on continuous fluidics. In this research program, we are combing programmable electrical actuation with nanofluidics for simpler and faster analysis of single molecules.